Co-infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other viruses has been reported.We evaluated cell lines commonly used to isolate viruses and diagnose related diseases for their susceptibility to SARS-CoV-2.Although multiple kidney cell lines from monkeys were susceptible to SARS-CoV-2, we found many cell types derived from humans, dogs, minks, cats, mice, and chicken were not.We analyzed MDCK cells, which are most commonly used for surveillance and study of u11-200ps influenza viruses, and found that they were not susceptible to SARS-CoV-2.The low expression level of the angiotensin converting enzyme 2 receptor and lower receptor affinity to SARS-CoV-2 spike, which could be overcome by overexpression of canine angiotensin converting enzyme 2 in trans, strengthened the cellular barrier to productive infection.
Moreover, emtek 2113 a D614G mutation in the spike protein did not appear to affect SARS-CoV-2 cell tropism.Our findings should help avert inadvertent propagation of SARS-CoV-2 from diagnostic cell lines.